Journal: Graefe's Archive for Clinical and Experimental Ophthalmology

Article Title: A rabbit model for outer retinal atrophy caused by surgical RPE removal

doi: 10.1007/s00417-023-06014-3

Figure Lengend Snippet: List of markers used for immunohistochemistry

Article Snippet: Pan cytokeratin , 1:100 , Bio-Techne, Wiesbaden, Germany , NBP2-44,368–0.1 mg , Monoclonal antibody, Clone PAN-CK , Epithelial cells, RPE.

Techniques: Concentration Assay, Plasmid Preparation

Journal: Graefe's Archive for Clinical and Experimental Ophthalmology

Article Title: A rabbit model for outer retinal atrophy caused by surgical RPE removal

doi: 10.1007/s00417-023-06014-3

Figure Lengend Snippet: Visualization of the treated area, the transition zone, and untreated area as direct comparison by SD-OCT and histology at post-operative day 4. Hematoxylin and eosin (HE) staining already reveals atrophy of the photoreceptor outer segments and the outer nuclear layer (ONL) limited to the scraping site. The inner nuclear layer (INL) is not affected. Immunohistochemistry staining of the rabbit retina shows proliferating cells detected via Ki67 staining (red) which colocalize in part with subretinal microglia/macrophages shown by isolectin B4 staining (yellow). A strong deposition of collagen IV (red) is found around the scraping site beneath the ONL. Pan cytokeratin staining (red) for RPE already appears at the scraping site. Cell nuclei are stained with DAPI (cyan). Scale bar for SD-OCT = 200 µm, for histological images = 100 µm

Article Snippet: Pan cytokeratin , 1:100 , Bio-Techne, Wiesbaden, Germany , NBP2-44,368–0.1 mg , Monoclonal antibody, Clone PAN-CK , Epithelial cells, RPE.

Techniques: Staining, Immunohistochemistry

Journal: Annals of Translational Medicine

Article Title: Macrophage regulator of G-protein signaling 12 contributes to inflammatory pain hypersensitivity

doi: 10.21037/atm-20-5729

Figure Lengend Snippet: Conditional RGS12 KO in mice inhibits the development of CAIA. (A) CAIA was induced in LysM-Cre+ and LysM-Cre+;RGS12fl/fl mice by injection 5 mg of a mAb mixture (Chondrex #53100) suspended in sterile PBS and subsequent challenge with 50 μg of LPS on day 3. At 9 days after immunization, the mice were sacrificed. (B) The paws of LysM-Cre+ and LysM-Cre+;RGS12fl/fl mice were immunized with 5 mg of a mAb mixture for 9 days as described in (A). Note that LysM-Cre+;RGS12fl/fl mice showed less swelling in the ankles (yellow double arrow) than LysM-Cre+ mice. (C) Ankle widths and (D) arthritis clinical scores of LysM-Cre+ and LysM-Cre+;RGS12fl/fl mice were measured and evaluated every day for 9 days. Statistically significant differences (***, P<0.001) were observed between LysM-Cre+ and LysM-Cre+;RGS12fl/fl mice. The data are expressed as the means ± SEM. (E) Hematoxylin and eosin (H&E) staining of RA synovial tissue samples from mice as described in (A) and (B). H&E staining showed a decrease in local inflammatory cell infiltration (yellow arrows) within synovial tissue in the LysM-Cre+;RGS12fl/fl group (B, bone area). ***, P<0.001, n=5. Scale bar: 500 mm.

Article Snippet: Collagen antibody-induced arthritis (CAIA) CAIA was induced in LysM-Cre + and LysM-Cre + ;RGS12 fl/fl mice (C57BL/6 background, females, 8 weeks old, n=5/cage) using a mixture of 5 mg of mAbs (Chondrex #53100) according to the manufacturer’s instructions.

Techniques: Injection, Sterility, Staining